A) Overexpression of GNG4 in LN229 cells – top: protein level, bottom: RNA level, right: Number of proliferating (viable) cells for LN229/Vector and LN229/GNG4 conditions measured every 24 hours for ten days, B) Colony formation capacity of GBM cells, C) Role of GNG4 in inhibition of transformation of immortalized normal human astrocytes (NHA-hTERT/E6/E7) by RAS V12 oncogene. p-value was calculated by Student’s t test where *, ** and *** represents p-value of < 0.05, < 0.01 and < 0.001 respectively.