A) Regulation of CXCR4 signaling by GNG4 tested by measuring the levels of phospho-Erk1/2 and phospho-Jnk, B) Regulation of CXCR4 downstream AKT pathway by GNG4 tested by measuring the levels of phosphorylation of Ser473 of Akt, C) Scratch assay in U87MG cell lines where CXCR4 signaling was activated using exogenous purified SDF1α and simultaneously GNG4 was overexpressed. Right: Quantification of scratch assay, D) Migration of U87MG cells tested using Boyden chamber assay. CXCR4 was activated using purified SDF1α. In the same condition, GNG4 was ectopically overexpressed to understand the effect of GNG4 in CXCR4 mediated migration of GBM cell lines. Right: Quantification of Boyden chamber assay. p-value was calculated by Student’s t test where *, ** and *** represents p-value of < 0.05, < 0.01 and < 0.001 respectively.