A tumor specific antibody to aid breast cancer screening in women with dense breast tissue
Lopamudra Das Roy1,2, Lloye M. Dillon1,2, Ru Zhou2, Laura J. Moore2, Chad Livasy3,4, Joe M. El-Khoury5, Rahul Puri1, Pinku Mukherjee1,2
1 OncoTAb, Inc., Charlotte, NC, USA
2 University of North Carolina at Charlotte, Charlotte, NC, USA
3 Carolinas Pathology Group, Carolinas Medical Center, Charlotte, NC, USA
4 University of North Carolina at Chapel Hill, Charlotte, NC, USA
5 Yale University, New Haven, CT, USA
Correspondence:
Pinku Mukherjee, email:
Keywords: tMUC1, Breast Cancer, Dense Breast, TAB 004 antibody
Received: April 20, 2017 Accepted: May 12, 2017 Published: May 21, 2017
Copyright: Roy et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC-BY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Screening for breast cancer has predominantly been done using mammography. Unfortunately, mammograms miss 50% cancers in women with dense breast tissue. Multi-modal screenings offer the best chance of enhancing breast cancer screening effectiveness. We evaluated the use of TAB004, an antibody that recognizes the tumor form of the glycoprotein MUC1 (tMUC1), to aid early detection of breast cancer. Our experimental approach was to follow tMUC1 from the tissue into circulation. We found that 95% of human breast cancer tissues across all subtypes stained positive for TAB004. In breast cancer cell lines, we showed that the amount of tMUC1 released from tumor cells is proportional to the cell’s tMUC1 expression level. Finally, we showed that TAB004 can be used to assess circulating tMUC1 levels, which when monitored in the context of cancer immunoediting, can aid earlier diagnosis of breast cancer regardless of breast tissue density. In a blinded pilot study with banked serial samples, tMUC1 levels increased significantly up to 2 years before diagnosis. Inclusion of tMUC1 monitoring as part of a multi-modal screening strategy may lead to earlier stage diagnosis of women whose cancers are missed by mammography.
